111In oxine-labeled rabbit platelets: in vivo distribution and sites of destruction.

نویسندگان

  • R L Hill-Zobel
  • U Scheffel
  • P A McIntyre
  • M F Tsan
چکیده

We have studied the kinetics, biodistribution, and fate of autologous platelets labeled with 111In-oxine in rabbits. The initial recovery was 75% and mean survival time was 2.8 days when the data were analyzed by the multiple-hit gamma function model. Using a modified geometric mean for correction of attenuation, there was good correlation between the values obtained by in vivo quantification and those obtained by postmortem measurements of the radioactivity in the liver and the spleen (i.e., r = 0.854 and 0.899, respectively, n = 32). Using this method, it was shown that after infusion, the 111In-platelets rapidly accumulated in these two organs reaching 35% and 12% of the injected dose in the liver and spleen, respectively, by 1 day. Thereafter, there was little subsequent change. On the sixth day, when essentially all of the 111In-platelets had cleared from the circulation, a total of 82% of the injected dose was deposited in the three major reticuloendothelial organs: liver (40%), spleen (14%), and bone marrow (28%). Our results suggest that in addition to liver and spleen, bone marrow played an important role in sequestering platelets in rabbits.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

“ In - Oxine - Labeled Rabbit Platelets : In Vivo Distribution and Sites of Destruction

We have studied the kinetics. biodistribution, and fate of autologous platelets labeled with 111ln-oxine in rabbits. The initial recovery was 75% and mean survival time was 2.8 days when the data were analyzed by the multiple-hit gamma function model. Using a modified geometric mean for correction of attenuation. there was good correlation between the values obtained by in vivo quantification a...

متن کامل

Subcellular localization of 111In in human and rabbit platelets.

In order to examine the subcellular distribution of 111In in 111In-oxine-labeled human and rabbit platelets, we employed a hypothetical grain technique of EM autoradiography analysis. The results indicate that in the rabbit 111In was concentrated within the platelet dense bodies, particularly when the platelets had been labeled in a plasma-free system. Under comparable conditions of labeling, h...

متن کامل

Subcellular Localization of “In in Human and Rabbit Platelets

In order to examine the subcellular distribution of 111In in “In-oxine-labeled human and rabbit platelets. we employed a hypothetical grain technique of EM autoradiograph analysis. The results indicated that in the rabbit ‘11In was concentrated within the platelet dense bodies. particularly when the platelets had been labeled in a plasma-free system. Under comparable conditions of labeling. hum...

متن کامل

Scintigraphic detection of carotid atherosclerosis with indium-111-labeled autologous platelets.

Using autologous platelets labeled with indium-111-oxine, we studied the localization of platelets on arterial lesions by radionuclide scintigraphy in 34 patients with suspected cerebrovascular disease. The imaging results were compared with the findings of contrast angiography in 23 patients, 16 of whom were receiving antiplatelet and/or anticoagulant drugs during the platelet imaging study. A...

متن کامل

A quantitative method to measure human platelet chemotaxis using indium-111-oxine-labeled gel-filtered platelets.

Human blood platelets have been shown to migrate directionally and specifically toward collagen in plasma in vitro. We have developed a new system to monitor this behavior using a linear 7-compartment chamber with 111In-oxine-labeled gel-filtered platelets. The compartments are separated by various Nuclepore and Millipore filter membranes. Radiolabeled platelets suspended in plasma are placed i...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:
  • Blood

دوره 61 1  شماره 

صفحات  -

تاریخ انتشار 1983